Deepyan Chatterjee ✉
Supervisor: Assoc. Prof. Ian Cockburn (ANU)
2nd Supervisor: Prof. Dr. Leif-Erik Sander Charité - Universitätsmedizin Berlin Med. Klinik m.S. Infektiologie und Pneumologie;
Immunization studies conducted with the repetitive section of Circumsporozoite protein (CSP) shows promising prospects, however it fails elicit long lasting memory response. Also the underlying mechanism of the humoral response against the immunodominant epitope still remains to be fully elucidated. The PhD project aims to decipher the cause of the immunodominance of the multivalent repeat section of CSP, and employ some of the finding for improving the existing vaccine strategies.
Jorin Diemer ✉
Main Supervisor: Prof. Dr. Dr. h.c. Edda Klipp, Humboldt-Universität zu Berlin (HU) Department of Biology;
2nd Supervisor: Prof. Kiaran Kirk (Australian National University, ANU)
In this project we model different aspects of interactions between Plasmodium falciparum and the infected red blood cell with a focus on lipid metabolism and ion homeostasis of the erythrocyte before and after parasite infection. Interference with lipid metabolism is known to affect viability of the malaria parasite. Consequently, the identification of new ways to intervene with the parasite's lipid metabolism could lead to new potential drug targets. After parasite infection, ion homeostasis of the red blood cell changes drastically, mainly due to novel permeability pathways emerging in the erythrocyte's membrane. Analyzing the interplay between host- and parasite-derived ion transportways will provide new insights into regulatory mechanisms of ion transport inhibitors that have recently emerged as potential antimalarial drugs.
Ayman Hemasa ✉
Main Supervisor: Kevin Saliba PhD (ANU); 2nd Supervisor: Prof. Dr. Frank Mockenhaupt, Charité - Universitätsmedizin Berlin Institute of Tropical Medicine and International Health
The intraerythrocytic stage of the human malaria parasite Plasmodium falciparum has unique vitamin requirements. Whilst progress has been made in our understanding of the parasite’s requirement for certain vitamins (e.g. pantothenate and thiamine), very little is known about the parasite’s requirement for other vitamins and the enzyme cofactors they are metabolised into. This project will investigate the parasite’s requirement for riboflavin (vitamin B2) with a view of determining whether riboflavin metabolism can serve as an antimalarial drug target.
Will Hirst ✉
Supervisor: Dr. Simone Reber, HU IRI Life Sciences;
2nd Supervisor: Prof. Dr. Kai Matuschewski, HU Department of Biology
Microtubules play a fundamental role in mitosis, intracellular transport, and establishing the structure of eukaryotic cells, and many diseases are treated with drugs that disrupt microtubule function. Using a recently developed affinity chromatography-based method, we have for the first time purified native tubulin from Plasmodium falciparum, which will allow us to perform the microtubule reconstitution assays central to microtubule research using this protein. I aim to characterize Plasmodium microtubule biochemistry and dynamics in vitro, and to quantitatively measure the direct effects of microtubule-disruptive drugs on Plasmodium microtubules in vitro and in vivo.
Julia Jäger ✉
Main Supervisor: Prof. Dr. Frank Mockenhaupt, Charité - Universitätsmedizin Berlin Institute of Tropical Medicine and International Health;
2nd Supervisor: Gaétan Burgio PhD (ANU)
The project aims to find polymorphisms in genes involved in iron regulation (e.g. SLCIIA2, SLC40A1, TF, DMT1) that are abundant in populations living in malaria-endemic regions. To confirm the protective effect of the polymorphisms it will be related to the odds of various entities and syndromes of malaria. Monoclonal antibodies and siRNA techniques will be used to identify pathways involved in potentially modified recognition and activation processes. Surface variation of infected RBCs will be identified by high resolution scanning microscopy and analysis of membrane components and adhesion properties will be done in collaboration with the Maier lab. Identified relevant polymorphisms will be tested for significance by using CRISPR/Cas9-mediated genome editing technology of erythroblasts.
Parnika Mukherjee ✉
Main Supervisor: Prof. Dr. Emanuel Heitlinger, HU & Leibniz Institute for Zoo and Wildlife Research;
2nd Supervisor: Gaétan Burgio PhD (ANU)
Understanding host-pathogen interactions is key to understanding infection mechanisms. In malaria, little is known about genes and proteins involved in these interactions. I am studying the effects of perturbations in gene regulatory networks and of correlated gene expression on infection mechanisms, first, using online databases and second, using gene expression data of mouse models.
Merryn Fraser ✉
Main Supervisor: Assoc. Prof. Dr. Alexander Maier (ANU);
2nd Supervisor: Prof. Dr. Dr. h.c. Edda Klipp (HU)
The survival of P. falciparum parasites relies on the metabolism of lipids. The aim of this project is to explore methods of interfering with this metabolism to decrease either the survival or virulence of these parasites. I will investigate drugs and processes known to affect metabolism, and identify other key lipid pathways by utilising both biochemical and theoretical modelling approaches. This research provides new insights into malaria treatment and prevention strategies.
Julie-Anne Gabelich ✉
HU Student Rep
Main Supervisor: Alyssa Ingmundson PhD, HU Department of Biology;
2nd Supervisor: Melanie Rug PhD (ANU)
Plasmodium parasites remodel their host cells to create an environment conducive to their development. This project investigates Plasmodium proteins present in the remodeled membranes at the interface between the parasite and host in both liver and blood cells. Understanding these membrane structures and the functions of these proteins will elucidate key mechanisms by which parasites influence their host environments. The project will use genetics, biochemistry and advanced microscopy to investigate the function of membrane structures generated by Plasmodium in their liver and erythrocyte host cells.
Stephanie Henkel ✉
Main Supervisor: Prof. Dr. Frank Seeber, Robert Koch-Institut, Berlin;
2nd Supervisor: Prof. Dr. Christian Schmitz-Linneweber (HU)
The overall aim of this project is to analyze the role of the small apicoplast-resident protein ferredoxin (Fd) in the different metabolic pathways it has been implicated in. Fd provides electrons to several important enzymes (LipA, LytB, GcpE) via protein-protein interactions (PPI), which we want to disrupt by cyclic peptides (dissociators). Identifying these dissociators by a genetic screen in E. coli and validating their specificity by biophysical binding studies and in situ expression in the apicoplast will be one aim of this project. In addition, an inducible Fd gene knockout will be attempted in Plasmodium falciparum using a conditional chemical rescue scheme. The resulting phenotypes in these transgenic parasite lines will be determined with a focus on parasite replication and growth, morphology, and biochemical profiles (e.g. fatty acid or isoprenoid content). Since a previous report implied a role of Fd in artemisinin resistance this can be validate by complementation of the Fd knockout with a set of Fd mutants.
Francois Korbmacher ✉
Main Supervisor: Prof. Dr. Kai Matuschewski, HU Department of Biology;
2nd Supervisor: Assoc. Prof. Dr. Alexander Maier (ANU)
Membrane transport proteins (MTPs) transport nutrients, metabolic products and inorganic ions across biological membranes. Although they are essential for the cell physiology of Plasmodium parasites, the actual functions of many transport proteins are still insufficiently clarified. Thus, functional analyses of MTPs are of great importance, not only due to their influence on the development of drug resistances, but also as possible targets in the development of active pharmaceutical ingredients. The aim of this work is to analyse a selection of MTPs in Plasmodium and to compare their importance, conservation, occurrence and function in the life cycle of Apicomplexan species.
Kai Pohl ✉
HU Student Rep
Main Supervisor: Prof. Dr. Leif-Erik Sander Charité - Universitätsmedizin Berlin Med. Klinik m.S. Infektiologie und Pneumologie;
2nd Supervisor: Assoc. Prof. Ian Cockburn (ANU)
High-throughput CRISPR/Cas9-genome engineering will be used to identify and characterize DC-Plasmodium sensing pathways, which will then be validated together with our Australian partners from the Cockburn lab. New insights may play a role in informed vaccine or adjuvant development against malaria.
Birte Steinhöfel ✉
Main Supervisor: Prof. Dr. Christian Schmitz-Linneweber, HU Department of Biology;
2nd Supervisor: Giel van Dooren PhD (ANU)
Analysis of heptatricopeptide repeat proteins in Toxoplasma
In the course of the project a novel family of apicomplexan organellar RNA binding proteins will be studied. Initial evidence suggests that they function in RNA stabilization, processing and ribosome assembly. As a genetically tractable model for Plasmodium biology, the role of these proteins in organellar RNA metabolism will be investigated in Toxoplasma gondii using a combination of RNA biochemistry and experimental genetics.
The Australian National University
Research School of Biology
134 Linnaeus Way
Canberra - Acton ACT 2601
Humboldt-Universität zu Berlin
Unter den Linden 6